Raman spectra of the GFP-like fluorescent proteins

GFP-like proteins stably accumulate in lysosomes.

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria, its GFP variants (Aequorea GFPs), and more recently the novel GFP-like proteins from Anthozoa have greatly advanced our technologies for fluorescently labeling cells, organelles, and proteins. It has been shown, however, that some GFP-like proteins have a tendency to oligomerize and aggregate. Transfection of GFP-like protein...

Alternative Cyclization in GFP-like Proteins Family

Computer Modeling of the Structure and Spectra of Fluorescent Proteins

Fluorescent proteins from the family of green fluorescent proteins are intensively used as biomarkers in living systems. The chromophore group based on the hydroxybenzylidene-imidazoline molecule, which is formed in nature from three amino-acid residues inside the protein globule and well shielded from external media, is responsible for light absorption and fluorescence. Along with the intense ...

Usefulness of Fluorescent Proteins: Gfp, Dsred and Fruit Fluorescent Proteins Inidentifying Multisynaptic Neuronal Chains. History and Our Own Experience

Investigation of the neuronalconnectionshave been conducted over a long span of time. Debate between researchers about contiguity or continuity of the nerve elements resulted in a new era in the research of neuronal pathways. The issue has been resolved when synaptic connections were discovered by the electron microscope. This made it possible to use two types of tract tracing methods. First no...

Mechanistic diversity of red fluorescence acquisition by GFP-like proteins.

This review aims to summarize our current state of knowledge of several post-translational modification mechanisms known to yield red fluorescence in the family of GFP-like (green fluorescent protein-like) proteins. We begin with a brief review of the maturation mechanism that leads to green fluorescence in GFPs. The main body of this article is focused on a series of main chain redox and beta-...